Search results for "kolonnu hromatogrāfija"

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Penicillium lanoso-viride AMP dezamināzes attīrīšana, izmantojot kolonnu hromatogrāfiju

2016

Darba mērķis bija iegūt pēc iespējas attīrītākas AMP dezamināzes frakcijas no mikroskopiskās sēnes Penicillium lanoso-viride glikoproteīnu preparātiem, izmantojot kolonnu hromatogrāfijas metodes, un raksturot iegūtās frakcijas. Mērķa sasniegšanai tika veikta hromatogrāfija DEAE celulozes un AMP sefarozes hromatogrāfijas kolonnās, kā arī attīrīšana, inkubējot proteīnu preparātus mēģenēs, kas pildītas ar DEAE celulozi. Pēc attīrīšanas ar DEAE celulozes hromatogrāfijas kolonnu tika iegūta aptuveni 3.7 reizes attīrītāka AMP dezamināzes frakcija, bet ar AMP sefarozes hromatogrāfijas metodi izdevās iegūt AMP dezamināzi ar attīrīšanas pakāpi 2.37 reizes. Ar DEAE celulozi pildītās mēģenēs AMP dezam…

AMP sefarozeAMP dezamināzeDEAE celulozeBioloģijaproteīnu attīrīšanakolonnu hromatogrāfija

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PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
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LSLife Sciences
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SHSocial Sciences and Humanities
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